do Valle G E, Zucchi M I, Stabellini N S, Lourenção A L, Pinheiro J B
Instituto Agronômico de Campinas, Campinas, SP, Brasil.
Neotrop Entomol. 2011 Mar-Apr;40(2):204-11. doi: 10.1590/s1519-566x2011000200008.
We aimed to characterize the population genetic structure within and among five Bemisia tabaci (Gennadius) populations collected from different host plants and geographic regions by using microssatelites as a molecular marker. Each population was represented by 19 specimens. The host plants and geographic origins of these populations were described as follows: Pop 1: Squash Barreiras (BA); Pop 2: Cotton Barreiras (BA); Pop 3: Soybean Campinas (SP); Pop 4: Tomato Cruz das Almas (BA); and Pop 5: Soybean Rondonópolis (MT). Six polymorphic loci were observed, which discriminated 31 different alleles in the studied populations, with a mean number of alleles per population of 3.30 (2.67 - 4.00). Using Fisher's Exact test, it was observed that at least three populations were in Hardy-Weinberg equilibrium for most of the studied loci (six). The dendrogram (UPGMA) separated populations into groups mainly related to the geographic origin of the samples. Only population 5 differed from the others at a 0.15 distance (74.5% group consistency). The most similar populations were 1 and 2, with a 0.01 distance (65.3%). This is in agreement with their geographic origins and it was not consistent with host specificity. The results suggest considerable gene flow (7.3%) among all whitefly populations and indicate that a better understanding of the gene flow in populations of B. tabaci associated with different hosts is required for the management of this insect.
我们旨在通过使用微卫星作为分子标记，来表征从不同寄主植物和地理区域收集的五个烟粉虱（Gennadius）种群内部和之间的群体遗传结构。每个种群由19个样本代表。这些种群的寄主植物和地理来源描述如下：种群1：来自巴雷里阿斯（BA）的南瓜；种群2：来自巴雷里阿斯（BA）的棉花；种群3：来自坎皮纳斯（SP）的大豆；种群4：来自克鲁斯达斯阿尔马斯（BA）的番茄；种群5：来自朗多诺波利斯（MT）的大豆。观察到六个多态性位点，在所研究的种群中区分出31个不同的等位基因，每个种群的等位基因平均数为3.30（2.67 - 4.00）。使用费舍尔精确检验，观察到对于大多数所研究的位点（六个），至少有三个种群处于哈迪-温伯格平衡。聚类图（UPGMA）将种群分为主要与样本地理来源相关的组。只有种群5在0.15的距离处与其他种群不同（群体一致性为74.5%）。最相似的种群是种群1和种群2，距离为0.01（群体一致性为65.3%）。这与它们的地理来源一致，并且与寄主特异性不一致。结果表明所有粉虱种群之间存在相当大的基因流（7.�%），并表明为了管理这种昆虫，需要更好地了解与不同寄主相关的烟粉虱种群中的基因流。
