Valle G E, Lourenção A L, Zucchi M I, Pinheiro J B
Seção de Entomologia, Centro de Fitossanidade, Instituto Agronômico de Campinas, Campinas, SP, Brasil.
Genet Mol Res. 2012 Nov 12;11(4):3899-903. doi: 10.4238/2012.November.12.7.
The silverleaf whitefly (Bemisia tabaci) is actually a complex of morphologically indistinct species that vary in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The worldwide impact of this whitefly has increased greatly, as it is a vector of various types of phytovirus, especially geminiviruses, in plants of economic importance. The adaptability of B. tabaci to many regions of the world has fostered the appearance of various biotypes that attack a broad spectrum of host plants. We developed microsatellite markers to study genetic variability and population structure of this whitefly in Brazil. Thirteen polymorphic microsatellite markers were isolated and characterized in 20 individuals from a natural population that were collected in soybean in Campinas (SP). The number of alleles per locus ranged from one to two, and the expected heterozygosity ranged from 0.000 to 0.505. These microsatellite markers will be useful for studies and management of B. tabaci. The low polymorphism found in these molecular markers is probably associated with homology of genes expressed in these markers.
烟粉虱（Bemisia tabaci）实际上是一个形态上难以区分的物种复合体，它们在传播植物病毒以及在具有经济重要性的植物中引发生理紊乱的能力方面存在差异。这种粉虱在全球范围内的影响大幅增加，因为它是多种植物病毒尤其是双生病毒在具有经济重要性的植物中的传播媒介。烟粉虱对世界许多地区的适应性促使出现了攻击广泛宿主植物的各种生物型。我们开发了微卫星标记来研究这种粉虱在巴西的遗传变异性和种群结构。从坎皮纳斯（圣保罗州）大豆田中采集的一个自然种群的20个个体中分离并鉴定了13个多态性微卫星标记。每个位点的等位基因数从1到2不等，预期杂合度从0.000到0.505不等。这些微卫星标记将有助于烟粉虱的研究和管理。在这些分子标记中发现的低多态性可能与这些标记中表达的基因的同源性有关。
