Hamel W, Westphal M, Shepard H
UNIV HOSP EPPENDORF,DEPT NEUROL SURG,BRAIN TUMOUR BIOL LAB,MARTINISTR 52,W-2000 HAMBURG 20,GERMANY. GENENTECH INC,DEPT DEV BIOL,SAN FRANCISCO,CA 94080.
Int J Oncol. 1992 Nov;1(6):673-82. doi: 10.3892/ijo.1.6.673.
Growth factor/ligand interactions have been shown to play an important role in various malignancies. Expression of two differentially spliced forms of SCF was detected in 20 of 24 glioma cell lines by RT-PCR. Northern blot analysis revealed expression of the corresponding receptor, c-kit (11/24), as well as PDGF alpha-receptor (22/25 glioma cell lines), PDGF beta-receptor (22/25), TGF-alpha (13/24 ) and PDGF B/c-sis (7/16) expression. As determined by FACS analysis, expression of EGFR and p185HER2 was detected in 21 of 21 and 15 of 21 glioma cell lines, respectively. Four cell lines showed moderate EGFR overexpression (>90,000 receptors/cell) and in one cell line p 185HER2 expression exceeded EGFR levels. Loss of EGFR gene amplification during in vitro culturing was observed in 3 of 18 investigated cell lines by differential PCR. In summary, our work suggests the simultaneous activation of several different growth factor/receptor systems in human glioma cell lines.
生长因子/配体相互作用已被证明在各种恶性肿瘤中起重要作用。通过逆转录聚合酶链反应（RT-PCR）在24个胶质瘤细胞系中的20个中检测到两种不同剪接形式的干细胞因子（SCF）的表达。Northern印迹分析显示相应受体c-kit（11/24）以及血小板衍生生长因子α受体（25个胶质瘤细胞系中的22个）、血小板衍生生长因子β受体（22/25）、转化生长因子α（13/24）和血小板衍生生长因子B/c-sis（16个中的7个）的表达。通过荧光激活细胞分选（FACS）分析确定，在21个胶质瘤细胞系中的21个和21个中的15个中分别检测到表皮生长因子受体（EGFR）和p185HER2的表达。四个细胞系显示出中度的EGFR过表达（>90,000个受体/细胞），并且在一个细胞系中p185HER2水平超过EGFR水平。通过差异PCR在18个研究的细胞系中的3个中观察到体外培养期间EGFR基因扩增的缺失。总之，我们的工作表明在人胶质瘤细胞系中几种不同的生长因子/受体系统同时被激活。
