Hamel W, Westphal M, Shepard H M
Department of Developmental Biology, Genentech, Inc., South San Francisco, California.
J Neurooncol. 1993 May;16(2):159-65. doi: 10.1007/BF01324703.
The retinoblastoma susceptibility gene (RB), the prototype of the class of tumor suppressor genes, is inactivated in a number of human malignancies. We investigated a possible role of RB in human brain tumors. Immunoprecipitation revealed frequent loss of RB protein expression in glioma cell lines (8/24), which was accompanied by lack of RB encoded transcripts. Among seventeen primary brain tumors studied by Western blotting, loss of Rb protein expression was observed in WHO grade 3 and 4 gliomas (3/10). However, none of the low grade gliomas and the other primary brain tumors investigated lacked RB protein expression. These data suggest a role for RB in glial malignancy, and loss of Rb expression appears to be associated with glial tumor progression.
视网膜母细胞瘤易感基因（RB）作为肿瘤抑制基因类别的原型，在多种人类恶性肿瘤中失活。我们研究了RB在人类脑肿瘤中的可能作用。免疫沉淀显示，胶质瘤细胞系中RB蛋白表达频繁缺失（8/24），同时伴有RB编码转录本的缺失。在通过蛋白质印迹法研究的17例原发性脑肿瘤中，WHO 3级和4级胶质瘤中观察到Rb蛋白表达缺失（3/10）。然而，所研究的低级别胶质瘤和其他原发性脑肿瘤均未出现RB蛋白表达缺失。这些数据表明RB在胶质细胞恶性肿瘤中发挥作用，且Rb表达缺失似乎与胶质细胞瘤进展相关。
