Department of Swine Diseases, National Veterinary Research Institute, Puławy, Poland.
Vet Res Commun. 2011 Aug;35(6):337-43. doi: 10.1007/s11259-011-9478-6. Epub 2011 May 17.
The influence of age and maternal antibodies on the development and duration of postvaccinal antibody response against Glässer's disease were investigated. Pigs born to immune (MDA-positive) and non-immune (MDA-negative) sows were vaccinated with inactivated vaccine. Vaccination was done according to three different protocols: at 1 and 4, at 2 and 5 or at 4 and 7 weeks of age. There were also two control groups for MDA-negative and MDA-positive pigs. The level of Haemophilus parasuis (Hps) specific antibodies were determined using commercial ELISA test. No serological responses were seen in any of the groups after the first vaccination. Maternally derived antibodies (MDA) against Hps were above the positive level until approximately 3 weeks of life in MDA-positive pigs. In those pigs the strongest postvaccinal humoral response was observed in piglets vaccinated at 4 and 7 weeks of age. In the remaining MDA-positive piglets only slight seroconversion was noted but levels of antibodies never exceeded values considered as positive. All MDA-negative pigs produced Hps-specific antibodies after the second vaccination. The results of the present study indicated that MDA may alter the development and duration of active postvaccinal antibody response. Age of pigs at the moment of vaccination was not associated with the significant differences in the magnitude of antibody response, however influenced the kinetics of decline of Hps-specific antibodies.
本研究旨在探讨年龄和母源抗体对猪格拉泽氏病（Glässer's disease）疫苗接种后抗体应答的发展和持续时间的影响。将出生于免疫（MDA 阳性）和非免疫（MDA 阴性）母猪的仔猪用灭活疫苗进行免疫接种。根据三种不同的方案进行接种：1 周龄和 4 周龄、2 周龄和 5 周龄或 4 周龄和 7 周龄。还设立了 MDA 阴性和 MDA 阳性猪的两个对照组。使用商业 ELISA 试验检测猪嗜血杆菌（Haemophilus parasuis，Hps）特异性抗体水平。首次免疫后，所有组均未出现血清学应答。在 MDA 阳性猪中，针对 Hps 的母源抗体（MDA）在生命的前 3 周内保持阳性水平。在这些仔猪中，在 4 周龄和 7 周龄接种的仔猪中观察到最强的疫苗接种后体液应答。在其余 MDA 阳性仔猪中仅观察到轻微的血清转化，但抗体水平从未超过被认为是阳性的水平。所有 MDA 阴性猪在第二次接种后均产生 Hps 特异性抗体。本研究结果表明，MDA 可能改变主动疫苗接种后抗体应答的发展和持续时间。接种时猪的年龄与抗体应答的幅度无显著差异相关，但影响 Hps 特异性抗体的下降动力学。
