Huhtala P, Eddy R L, Fan Y S, Byers M G, Shows T B, Tryggvason K
Biocenter, University of Oulu, Finland.
Genomics. 1990 Mar;6(3):554-9. doi: 10.1016/0888-7543(90)90486-e.
The complete amino acid sequence of the human type IV collagenase preproenzyme was determined from cDNA and genomic clones. Primer extension and S1 nuclease analyses as well as nucleotide sequencing of a genomic clone indicate that the first exon has two closely spaced initiation sites for transcription and codes for 290 and 280 nt of a 5' untranslated region and a 29-residue signal peptide. The gene (CLG4) was localized to 16q21 using somatic cell hybrids and in situ hybridization.
通过cDNA和基因组克隆确定了人IV型胶原酶前体酶原的完整氨基酸序列。引物延伸和S1核酸酶分析以及基因组克隆的核苷酸测序表明，第一个外显子有两个紧密间隔的转录起始位点，编码5'非翻译区的290和280个核苷酸以及一个29个残基的信号肽。利用体细胞杂种和原位杂交将该基因（CLG4）定位于16q21。
