Lusis A J, Zollman S, Sparkes R S, Klisak I, Mohandas T, Drayna D, Lawn R M
Department of Medicine, School of Medicine, University of California, Los Angeles 90024.
Genomics. 1987 Nov;1(3):232-5. doi: 10.1016/0888-7543(87)90049-8.
We have used a cDNA probe for human cholesteryl ester transfer protein (CETP) to determine the chromosomal location for the human gene. Southern blot analysis of DNA from 17 independent mouse-human somatic cell hybrids demonstrated the presence of the gene for human CETP on chromosome 16. Regional mapping of the gene by in situ hybridization was consistent with these results and indicated that the gene resides in the 16q12-21 region of the chromosome. These findings provide an additional polymorphic marker for chromosome 16, as several relatively common restriction fragment length polymorphisms of the gene have previously been reported, and they have significance for studies directed at the identification of genetic factors affecting plasma lipoprotein metabolism and atherosclerosis.
我们使用了一种针对人类胆固醇酯转运蛋白（CETP）的cDNA探针来确定该人类基因的染色体定位。对来自17个独立的小鼠-人类体细胞杂种的DNA进行的Southern印迹分析表明，人类CETP基因位于16号染色体上。通过原位杂交对该基因进行区域定位与这些结果一致，并表明该基因位于染色体的16q12 - 21区域。这些发现为16号染色体提供了另一个多态性标记，因为此前已经报道了该基因的几种相对常见的限制性片段长度多态性，并且它们对于旨在鉴定影响血浆脂蛋白代谢和动脉粥样硬化的遗传因素的研究具有重要意义。
