Song Wenling, Liu Xiaozhen, Cai Xinzhi, Sun Di, Dai Chuanchao
Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(3):302-6.
The effect of the endophytic fungi Botrytis sp. (C1) or Chaetomium globosum (C4) on the drought resistance of Chrysanthemum morifolium was studied. Ch. morifolium plantlets were inoculated with C1, C4 and cultured in the pots for 60 days, then the plantlets were stressed by 0%, 10%, 20%, 30%, 40% PEG6000 respectively in order to simulate different drought conditions. Biomass, the activities of SOD, POD, PAL, the contents of MDA and soluble protein of each group were determined. The results showed that endophytic fungi groups grew better than the control (without inoculation endophytic fungi). With the increasing of the concentration of PEG6000, the biomass of Ch. morifolium of each groups decreased, while the biomass of fungi groups was significantly higher than that of control, moreover C4 group higher than C1 group. With the concentration of PEG increasing, the content of MDA of each group increased too, while POD activity and soluble protein content of all treatments increased at first and then decreased. SOD activity and PAL activity of the control were increased with the increase of PEG concentration, but SOD activity of the two fungi groups were stable. After been stressed by different concentrations of PEG, MDA content of two fungi groups were always lower than the control, while SOD activity, POD activity, PAL activity and soluble protein content were higher. In conclusion, endophytic fungi can increase the drought resistance of Ch. morifolium.
研究了内生真菌灰葡萄孢（C1）或球毛壳菌（C4）对菊花抗旱性的影响。将菊花幼苗接种C1、C4并在花盆中培养60天，然后分别用0%、10%、20%、30%、40%的聚乙二醇6000（PEG6000）对幼苗进行胁迫，以模拟不同的干旱条件。测定了各组的生物量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）活性、丙二醛（MDA）和可溶性蛋白含量。结果表明，内生真菌组生长状况优于对照组（未接种内生真菌）。随着PEG6000浓度的增加，各组菊花的生物量均下降，但真菌组生物量显著高于对照组，且C4组高于C1组。随着PEG浓度的增加，各组MDA含量也增加，而所有处理的POD活性和可溶性蛋白含量先升高后降低。对照组的SOD活性和PAL活性随PEG浓度的增加而升高，但两个真菌组的SOD活性稳定。经不同浓度PEG胁迫后，两个真菌组的MDA含量始终低于对照组，而SOD活性、POD活性、PAL活性和可溶性蛋白含量均较高。综上所述，内生真菌可提高菊花的抗旱性。
