Yu Xiao-feng, Xu Zhi-jian, Zhang Sheng-qin, Lv Si-jie, Fu You-zhang, Hong Sen-rong, Yin Ming-hua
Zhong Yao Cai. 2016 Jan;39(1):11-5.
In order to provide the theoretical basis for the Guangfeng medicinal yam( Dioscorea opposita) in field transplanting, the effect of PEG-6000 simulation drought stress on physiological characteristics of Guangfeng medicinal yam plantlets was studied.
Using the method of spectrophotometer,the content of total chlorophyll,soluble total sugar, soluble protein and praline,as well as the activities of SOD,CAT and POD of Guangfeng medicinal yam plantlets were tested under PEG-6000 treatment.
Under PEG-6000 simulated drought stress, with the increasing of drought stress and the extension of stress time, the total chlorophyll content of Guangfeng medicinal yam plantlets continued to decline, the content of total soluble sugar, proline and MDA of Guangfeng medicinal yam plantlets significantly increased, the content of soluble protein and the activities of CAT,POD and SOD of Guangfeng medicinal yam plantlets increased at first and then decreased.
This study reveals the changes of physiological indices of Guangfeng medicinal yam plantlets under PEG-6000 simulation drought stress, which indicated that Guangfeng medicinal yam plantlets have certain drought tolerance.
为广丰药用山药（薯蓣）田间移栽提供理论依据，研究聚乙二醇-6000（PEG-6000）模拟干旱胁迫对广丰药用山药组培苗生理特性的影响。
采用分光光度计法，测定PEG-6000处理下广丰药用山药组培苗的总叶绿素、可溶性总糖、可溶性蛋白和脯氨酸含量以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性。
在PEG-6000模拟干旱胁迫下，随着干旱胁迫程度的增加和胁迫时间的延长，广丰药用山药组培苗的总叶绿素含量持续下降，可溶性总糖、脯氨酸和丙二醛（MDA）含量显著增加，可溶性蛋白含量以及CAT、POD和SOD活性先升高后降低。
本研究揭示了PEG-6000模拟干旱胁迫下广丰药用山药组培苗生理指标的变化，表明广丰药用山药组培苗具有一定的耐旱性。
