Li Chun-hua, Zhao Ya-fang, Ji Bo, Ren Xiao-xuan, Guo Meng-wei, Ding Xi-yan, Sun Lin-lin, Zhang Lu-fen, Zhu Jiang
School of Acu-moxibustion and Tuina , Beijing University of Chinese Medicine, Beijing 100029, China.
Zhen Ci Yan Jiu. 2011 Feb;36(1):12-7.
OBJECTIVE To observe the effect of electroacupuncture (EA) on uterine microcirculation in dysmenorrhea rats so as to investigate its mechanism underlying relieving primary dysmenorrheal (PD).
Female SD rats undergoing diestrus were randomly divided into saline control, model, Sanyinjiao(SP 6), Xuanzhong(GB 39) and non-acupoint groups, with 6 rats in each. PD model was established by subcutaneous injection of estradiol benzoate for 10 days and intra-peritoneal injection of oxytocin (2 U) 1 h after the last estradiol injection. EA (2 Hz/100 Hz, 1- 1.5 mA) was applied to the above-mentioned acupoints and non-acupoint area for 20 minutes. Numbers and diameters of the uterine microvessels (11-100 microm) and capillaries (< or =10 microm), and the state of the uterine microcirculation were observed by using a Cold Light Microcirculation Detector.
In comparison with the saline control group, the numbers and diameters of uterine microvessels and capillaries at the corresponding time-points 5 min, 10 min and 20 min were decreased significantly in the model group (P<0. 05,P<0. 01). While compared with the model group, the numbers and diameters of uterine microvessels and capillaries at 20 min after EA in the SP 6 group were increased significantly (P<0. 05). The diameter of uterus capillaries of SP 6 group was significantly bigger than that of the non-acupoint group at the time-point 20 min (P<0. 05). No significant differences were found among the SP 6, GB 39 and non-acupoint groups in the diameter of uterine microvessels (P>0. 05).
EA of SP 6 can effectively increase the number of uterine microvessels and capillaries and the diameter of the uterine microvessels and capillaries in PD rats, which may contribute to its effect in relieving dysmenorrhea by improving uterine microcirculation.
目的 观察电针（EA）对痛经大鼠子宫微循环的影响，以探讨其缓解原发性痛经（PD）的机制。
将处于动情间期的雌性SD大鼠随机分为生理盐水对照组、模型组、三阴交（SP 6）组、悬钟（GB 39）组和非穴位组，每组6只。通过皮下注射苯甲酸雌二醇10天，并在最后一次注射雌二醇1小时后腹腔注射催产素（2 U）建立PD模型。将EA（2 Hz/100 Hz，1 - 1.5 mA）施加于上述穴位和非穴位区域20分钟。使用冷光微循环检测仪观察子宫微血管（11 - 100微米）和毛细血管（≤10微米）的数量和直径，以及子宫微循环状态。
与生理盐水对照组相比，模型组在相应时间点5分钟、10分钟和20分钟时子宫微血管和毛细血管的数量及直径均显著降低（P<0.05，P<0.01）。与模型组相比，SP 6组电针后20分钟时子宫微血管和毛细血管的数量及直径显著增加（P<0.05）。SP 6组在20分钟时间点子宫毛细血管直径显著大于非穴位组（P<0.05）。SP 6组、GB 39组和非穴位组在子宫微血管直径方面未发现显著差异（P>0.05）。
SP 6穴位电针可有效增加PD大鼠子宫微血管和毛细血管的数量以及子宫微血管和毛细血管的直径，这可能是其通过改善子宫微循环缓解痛经的作用机制。
