瓜纳卡斯特树 Enterolobium cyclocarpum 中微卫星位点的分离与特征分析

Isolation and characterization of microsatellite loci in the Guanacaste tree, Enterolobium cyclocarpum.

机构信息

Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA, Institute of Ecology, University of Georgia, Athens, GA 30602, USA, Department of Plant Biology, University of Georgia, Athens, GA 30602, USA, Department of Biological Sciences, Kent State University, Kent, OH 44242, USA, Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ 08901, USA.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):129-31. doi: 10.1111/j.1471-8286.2007.01896.x.

DOI:10.1111/j.1471-8286.2007.01896.x

PMID:21585735

Abstract

We isolated nine microsatellite loci from the Guanacaste tree (Enterolobium cyclocarpum) and optimized them for future research on breeding populations of this species. Loci were screened across 53 individuals from one population and were shown to be variable with the number of alleles per locus ranging from five to 15. Polymorphic information content ranged from 0.420 to 0.900 and observed heterozygosity ranged from 0.547 to 0.906.

摘要

我们从瓜纳卡斯特树（Enterolobium cyclocarpum）中分离出九个微卫星位点，并对其进行了优化，以便对该物种的繁殖群体进行未来的研究。在一个种群的 53 个个体中筛选出这些位点，结果表明这些位点具有变异性，每个位点的等位基因数从 5 到 15 不等。多态信息含量范围从 0.420 到 0.900，观察到的杂合度范围从 0.547 到 0.906。