一套用于入侵植物曼陀罗（伞形科）质体插入缺失和核微卫星的引物及其在曼陀罗属 Sphondylium 中的可转移性。
A set of primers for plastid indels and nuclear microsatellites in the invasive plant Heracleum mantegazzianum (Apiaceae) and their transferability to Heracleum sphondylium.
机构信息
Department of Ecology and Evolution, Biophore, UNIL, 1015 Lausanne, Switzerland, School of Biological Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK, Department of Ecology, Faculty of Science, Charles University, Vinicná 7, Prague 2, Czech Republic, and Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43 Pruhonice, Czech Republic.
出版信息
Mol Ecol Resour. 2008 Jan;8(1):161-3. doi: 10.1111/j.1471-8286.2007.01911.x.
This study reports the isolation and polymorphism characterization of four plastid indels and six nuclear microsatellite loci in the invasive plant Heracleum mantegazzianum. These markers were tested in 27 individuals from two distant H. mantegazzianum populations. Plastid indels revealed the presence of five chlorotypes while five nuclear microsatellite loci rendered polymorphism. Applications of these markers include population genetics and phylogeography of H. mantegazzianum. A very good transferability of markers to Heracleum sphondylium was demonstrated.
本研究报告了入侵植物曼陀罗中四个质体插入/缺失和六个核微卫星位点的分离和多态性特征。这些标记在来自两个遥远的曼陀罗种群的 27 个个体中进行了测试。质体插入/缺失揭示了存在五种叶绿素类型，而五个核微卫星位点呈现多态性。这些标记的应用包括曼陀罗种群遗传学和系统地理学。还证明了标记对 Sphondylium heracleifolium 的很好的可转移性。
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