Walasek Magdalena, Grzegorczyk Agnieszka, Malm Anna, Skalicka-Woźniak Krystyna
Department of Pharmacognosy with Medicinal Plants Unit, Medical University of Lublin, 1 Chodzki Str, 20-093 Lublin, Poland.
Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Str, 20-093 Lublin, Poland.
Food Chem. 2015 Nov 1;186:133-8. doi: 10.1016/j.foodchem.2015.02.011. Epub 2015 Feb 9.
An efficient strategy, based on bioassay-guided fractionation, high-performance liquid chromatography (HPLC), and high-performance counter-current chromatography (HPCCC), was established to purify and evaluate the bioactive compounds from the dichloromethane extract of the fruits of Heracleum mantegazzianum Sommier & Levier (Apiaceae). The quaternary solvent system n-heptane-ethyl acetate-methanol-water (6:5:6:5 v/v) was used in the reversed phase mode. Using this method, in a single run, seven fractions were isolated, among them three were the pure furanocoumarins: pimpinellin, imperatorin, and phellopterin. In order to purify xanthotoxin a more polar system (1:1:1:1 v/v) was further applied. The antimicrobial activity of extract, chromatographic fractions, and single compounds were in the range of MIC = 0.03-1 mg mL(-1). Xanthotoxin may have priority as a compound of further interest based on its antimicrobial activity. For the first time, an extensive antimicrobial study was performed for pimpinellin and phellopterin.
建立了一种基于生物活性导向分级分离、高效液相色谱（HPLC）和高效逆流色谱（HPCCC）的有效策略，用于从独活（伞形科）果实的二氯甲烷提取物中纯化和评估生物活性化合物。在反相模式下使用正庚烷 - 乙酸乙酯 - 甲醇 - 水（6:5:6:5 v/v）的四元溶剂系统。使用该方法，单次运行可分离出七个馏分，其中三个是纯呋喃香豆素：茴芹内酯、欧前胡素和异补骨脂素。为了纯化花椒毒素，进一步应用了极性更强的系统（1:1:1:1 v/v）。提取物、色谱馏分和单一化合物的抗菌活性范围为MIC = 0.03 - 1 mg mL(-1)。基于其抗菌活性，花椒毒素可能是更值得进一步研究的化合物。首次对茴芹内酯和异补骨脂素进行了广泛的抗菌研究。
