School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK, CSIRO Sustainable Ecosystems, Atherton, Qld 4883, Australia, Escuela de Biologia, Fac. Ciencias Naturales, Universidad Autonoma de Queretaro, CP 76010 Queretaro, Mexico.
Mol Ecol Resour. 2008 Jan;8(1):164-7. doi: 10.1111/j.1471-8286.2007.01913.x.
Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.
刺梨仙人掌在贸易中很常见，但在其自然栖息地已濒临灭绝。为了最终制定一个认证计划，以帮助保护这一物种，我们从非富集文库中分离出了刺梨仙人掌微卫星。57 个序列包含微卫星阵列，其中 12 个在来自单一野生种群的 30 个个体中具有多态性。在对 27 种其他仙人掌物种的筛选中，所有 12 对微卫星引物在一种或多种物种中扩增出产物。
