分离和鉴定加勒比海扇珊瑚病原菌 Sydow 曲霉中的微卫星位点。

Isolation and characterization of microsatellite loci in Aspergillus sydowii, a pathogen of Caribbean sea fan corals.

机构信息

Department of Ecology and Evolutionary Biology, Dale R. Corson Hall, Cornell University, Ithaca, NY 14853, USA.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):230-2. doi: 10.1111/j.1471-8286.2007.01934.x.

DOI:10.1111/j.1471-8286.2007.01934.x

Abstract

Here we report on nine microsatellite loci designed for Aspergillus sydowii, a widely distributed soil saprobe that is also the pathogenic agent of aspergillosis in Caribbean sea fan corals. Primers were tested on 20 A. sydowii isolates from the Caribbean, 17 from diseased sea fans and three from environmental sources. All loci were polymorphic and exhibited varying degrees of allelic diversity (three to nine alleles). Gene diversity (expected heterozygosity) ranged from 0.353 to 0.821. These primers will enable future research into the epidemiology of A. sydowii as an emergent infectious disease.

摘要

在这里，我们报告了九个微卫星基因座，这些基因座是为广泛分布于土壤中的腐生菌棘孢木霉（Aspergillus sydowii）设计的，棘孢木霉也是加勒比海扇珊瑚曲霉病的病原体。引物在来自加勒比海的 20 个棘孢木霉分离株、17 个来自患病海扇和 3 个来自环境来源的分离株上进行了测试。所有的基因座都是多态的，表现出不同程度的等位基因多样性（三到九个等位基因）。基因多样性（预期杂合度）范围从 0.353 到 0.821。这些引物将使未来对棘孢木霉作为一种新兴传染病的流行病学研究成为可能。