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永久性遗传资源:墨西哥林下棕榈 Chamaedorea elegans 的微卫星标记开发、种间基因分型以及近缘种的扩增。

PERMANENT GENETIC RESOURCES: Development of microsatellite markers of the Mexican understorey palm Chamaedorea elegans, cross-species genotyping, and amplification in congeners.

机构信息

Department of Ecology, Evolution, and Environmental Biology, Columbia University, 1200 Amsterdam Avenue, MC5557, New York, NY 10027, USA, Department of Biology, Georgetown University, 37th & O Streets, NW, Washington, D.C. 20057, USA, Division of Invertebrate Zoology and Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):322-4. doi: 10.1111/j.1471-8286.2007.01942.x.

Abstract

With striking morphological diversity and adaptability, Chamaedorea palms constitute an ecologically and economically important understorey component of Neotropical forests. Nine loci developed for Chamaedorea elegans evaluated in three Veracruz populations resulted in a large number of alleles (8-18), and high expected heterozygosity (0.49-0.92), but low observed (0.27-0.65) heterozygosity. Deviations from Hardy-Weinberg and high inbreeding suggest a lack of panmixia. Eight loci optimized for Chamaedorea ernesti-augustii showed similar patterns of variation. All nine multiplexing loci amplified in other five congeneric species, which will facilitate comparisons within the genus and contribute to the conservation of its genetic resources.

摘要

形态多样且适应性强的雀稗棕榈是新热带森林中具有重要生态和经济意义的林下组成部分。在维拉克鲁斯的三个种群中评估了为 Chamaedorea elegans 开发的九个基因座,结果产生了大量的等位基因(8-18 个)和高预期杂合度(0.49-0.92),但观察到的杂合度较低(0.27-0.65)。偏离 Hardy-Weinberg 平衡和高度近亲繁殖表明缺乏泛群集。为 Chamaedorea ernesti-augustii 优化的 8 个基因座显示出相似的变异模式。所有九个多重扩增位点在其他五个同属种中都能扩增,这将有助于在属内进行比较,并有助于保护其遗传资源。

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