开发并鉴定中国菊头蝠（Rhinolophus sinicus）的新型微卫星标记，这些标记在亲缘关系密切的分类群中具有跨物种扩增能力。

Development and characterization of novel microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) with cross-species amplification in closely related taxa.

机构信息

School of Life Science, East China Normal University, Shanghai 200062, China, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China, School of Biological and Chemical Sciences, Queen Mary, University of London, London E1 4NS, UK.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):183-5. doi: 10.1111/j.1755-0998.2008.02434.x. Epub 2008 Oct 21.

DOI:10.1111/j.1755-0998.2008.02434.x

PMID:21564597

Abstract

We isolated nine polymorphic microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) using an enriched library method. We assessed genetic polymorphism at these loci in 42 individuals from a single population. We recorded high genetic diversity with four to 17 alleles per locus, and estimated expected and observed heterozygosity values ranging from 0.492 to 0.910 and from 0.462 to 0.881, respectively. No locus departed from Hardy-Weinberg equilibrium following Bonferroni correction, and no linkage disequilibrium was detected. Most loci successfully cross-amplified congeneric species. These loci will be used to characterize phylogeographical history of Rhinolophus sinicus in China.

摘要

我们采用富集文库法从中华菊头蝠（Rhinolophus sinicus）中分离出九个多态性微卫星标记。我们在一个单一种群的 42 个个体中评估了这些位点的遗传多态性。我们记录了每个位点 4 到 17 个等位基因的高遗传多样性，并估计了从 0.492 到 0.910 和从 0.462 到 0.881 的预期和观察杂合度值。没有一个位点在经过 Bonferroni 校正后偏离哈迪-温伯格平衡，也没有检测到连锁不平衡。大多数位点成功地与同属物种进行了交叉扩增。这些标记将用于研究中国中华菊头蝠的系统地理历史。