永久遗传资源：黄花棘豆微卫星标记的开发与跨种扩增。

PERMANENT GENETIC RESOURCES: Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification.

机构信息

Biodiversity Research Centre, Unité d'écologie et de biogéographie, Université catholique de Louvain, Croix du Sud 4-5, 1348 Louvain-la-Neuve, Belgium, UMR 1098 CIRAD,TA A96/03 Avenue Agropolis, 34398 Montpellier cedex 5, France.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):384-6. doi: 10.1111/j.1471-8286.2007.01966.x.

DOI:10.1111/j.1471-8286.2007.01966.x

PMID:21585799

Abstract

Fifteen polymorphic microsatellite loci were developed from an enriched genomic library of the annual plant Rhinanthus angustifolius and characterized using 36 individuals. These markers provided high polymorphism ranging from two to 15 alleles per locus. Four loci showed significant departure from Hardy-Weinberg equilibrium, probably because of the occurrence of null alleles. No significant linkage disequilibrium was detected between pairs of loci. Tests of cross-species transferability were performed on four congeners with a success rate of 100% in Rhinanthus minor, 93% in R. mediterraneus and R. glacialis, and 80% in R. alectorolophus. These microsatellite loci will be useful tools to study mating system, gene flow and hybridization in the genus Rhinanthus.

摘要

从一年生植物米口袋（Rhinanthus angustifolius）的富集基因组文库中开发了 15 个多态性微卫星标记，并对 36 个个体进行了特征分析。这些标记表现出高度多态性，每个位点的等位基因数从 2 到 15 不等。其中 4 个位点表现出与哈迪-温伯格平衡显著偏离，可能是由于存在无效等位基因。在两个位点之间未检测到显著的连锁不平衡。在四个近缘种中进行了跨物种转移能力的测试，在米口袋（Rhinanthus minor）中成功率为 100%，在欧洲米口袋（R. mediterraneus）和冰川米口袋（R. glacialis）中成功率为 93%，在山米口袋（R. alectorolophus）中成功率为 80%。这些微卫星标记将成为研究米口袋属交配系统、基因流和杂交的有用工具。