US Geological Survey-Biological Resources Division, Leetown Science Center-Aquatic Ecology Branch, 11649 Leetown Road, Kearneysville, WV 25430, USA WV Cooperative Fish and Wildlife Research Unit, Biological Resources Division, US Geological Survey, West Virginia University, Morgantown, WV 26506-6125, USA.
Mol Ecol Resour. 2008 Mar;8(2):335-8. doi: 10.1111/j.1471-8286.2007.01946.x.
In order to investigate a potential hybrid zone between the candy darter, Etheostoma osburni, and variegate darter, Etheostoma variatum, and examine population variation within E. osburni, a suite of primers for 15 polymorphic microsatellite loci were developed. The average number of alleles per locus was 5.5 in E. osburni and 7.6 in E. variatum, and the average observed heterozygosities were 62.5% and 71.4%, respectively. There were no deviations from Hardy-Weinberg equilibrium and no observed linkage disequilibrium after Bonferroni correction. The utility of these primers was also tested in 11 species of darters representing all four genera of darters. Success of cross-species amplification was largely consistent with phylogenetic relationships of darters.
为了调查糖果弓背鱼（Etheostoma osburni）和杂斑弓背鱼（Etheostoma variatum）之间的潜在杂交区，并研究 E. osburni 内的种群变异，开发了一套用于 15 个多态性微卫星基因座的引物。每个基因座的平均等位基因数分别为 5.5 个在 E. osburni 和 7.6 个在 E. variatum，平均观察杂合度分别为 62.5%和 71.4%。经过 Bonferroni 校正后，没有偏离哈迪-温伯格平衡，也没有观察到连锁不平衡。这些引物在代表所有四个弓背鱼属的 11 种弓背鱼中也进行了测试。跨物种扩增的成功率与弓背鱼的系统发育关系基本一致。
