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23 个微卫星 DNA 位点用于橙喉拟石首鱼的群体遗传学研究和亲子关系鉴定。

Twenty-three microsatellite DNA loci for population genetic studies and parentage assignment in orangethroat darter, Etheostoma spectabile.

机构信息

Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA, Natural History Museum and Biodiversity Research Center, University of Kansas, Dyche Hall, 1345 Jayhawk Boulevard, Lawrence, KS 66045, USA, 1637 Merion Place, Lawrence, Kansas 66047, USA.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1483-5. doi: 10.1111/j.1755-0998.2008.02312.x. Epub 2008 Sep 3.

DOI:10.1111/j.1755-0998.2008.02312.x
PMID:21586083
Abstract

The genus Etheostoma is a species-rich and ecologically important group of fishes in North America. The orangethroat darter (Etheostoma spectabile) is widely distributed and abundant in headwater streams throughout the central Midwest, and is an excellent model for ecological and mating system studies. We developed 23 novel, polymorphic, and independent microsatellite loci for E. spectabile. We found from two to 14 alleles per locus, and observed heterozygosities ranged from 0.39 to 1.0. These markers, in combination with others isolated from Etheostoma taxa, will be useful for ecological and evolutionary studies in the genus.

摘要

鲈形目鮨科是北美的一个物种丰富且具有重要生态意义的鱼类群体。橙喉拟鲈广泛分布于中西部的溪流中,数量丰富,是生态和交配系统研究的理想模型。我们为橙喉拟鲈开发了 23 个新的、多态的和独立的微卫星位点。每个位点检测到 2 到 14 个等位基因,观测杂合度范围为 0.39 到 1.0。这些标记与从其他鮨科鱼类中分离的标记相结合,将有助于该属的生态和进化研究。

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