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十一组科利氏海鸠(Cory's shearwater)微卫星多态性标记及其在受威胁的管鼻类物种上的跨物种扩增

Eleven polymorphic microsatellite markers in Cory's shearwater, Calonectris diomedea, and cross-species amplification on threatened Procellariiformes.

机构信息

Departamento de Oceanografia e Pescas, Centro do IMAR da Universidade dos Açores, 9901-862 Horta, Açores, Portugal, CEFE, UMR 5175 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.

出版信息

Mol Ecol Resour. 2008 May;8(3):602-4. doi: 10.1111/j.1471-8286.2007.02009.x.

Abstract

For the first time, we describe 11 variable dinucleotide microsatellites and the conditions for multiplexing and simultaneous genotyping sets of loci in Cory's shearwater, Calonectris diomedea. Microsatellite variability was assessed in a colony from the Azores archipelago (Atlantic Ocean). Two to eight alleles were detected per locus, the mean gene diversity being 4.5. Cross-species amplification in three other seabirds (Diomedea exulans, Procellaria aequinoctialis and Bulweria bulwerii) revealed some variability at one, two and eight loci, respectively.

摘要

我们首次描述了 11 个可变二核苷酸微卫星,以及在紫水鸡(Calonectris diomedea)中对多套基因座进行多重复合扩增和同时基因分型的条件。我们在亚速尔群岛(大西洋)的一个繁殖地评估了微卫星的变异性。每个基因座检测到 2 到 8 个等位基因,平均基因多样性为 4.5。在另外三种海鸟(黑眉信天翁、北大西洋海雀和黑脚信天翁)中的跨物种扩增分别在 1、2 和 8 个基因座中显示出一些可变性。

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