鉴定并描述埃及血吸虫第一个多态性微卫星标记，并将其应用于幼虫期的多重反应。
Isolation and characterization of the first polymorphic microsatellite markers for Schistosoma haematobium and their application in multiplex reactions of larval stages.
机构信息
Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College, London, Norfolk Place, London W2 1PG, UK, Wolfson Wellcome Biomedical Laboratories, Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UK.
出版信息
Mol Ecol Resour. 2008 May;8(3):647-9. doi: 10.1111/j.1471-8286.2007.02031.x.
Abstract
The ability of microsatellite loci to reveal genetic diversity within the trematode Schistosoma haematobium is demonstrated for the first time. Nine novel polymorphic microsatellite markers were isolated and their viability assessed on 36 S. haematobium adult worm individuals from three geographical populations. Allelic diversity and gene diversity ranged from two to seven and from 0.29 to 0.76, respectively, suggesting high variability between individuals and between unrelated populations. Three primers also amplified Schistosoma mansoni and two Schistosoma japonicum. The results suggest these primers are useful for population genetic analyses of S. haematobium.
摘要
首次证明微卫星位点能够揭示曼氏血吸虫（Schistosoma haematobium）内部的遗传多样性。分离出 9 个新的多态性微卫星标记，并在来自三个地理种群的 36 个曼氏血吸虫成虫个体上评估其活力。等位基因多样性和基因多样性分别在 2 到 7 之间和在 0.29 到 0.76 之间，表明个体之间和无关种群之间存在高度的变异性。有三个引物也扩增出曼氏血吸虫和两个日本血吸虫。结果表明这些引物可用于曼氏血吸虫种群遗传分析。
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