Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK.
Mol Ecol Resour. 2008 Jul;8(4):736-41. doi: 10.1111/j.1755-0998.2007.02074.x.
DNA extraction techniques that employ the reversible binding of DNA to silica via chaotropic salts can deliver high-quality genomic DNA from plant and animal tissues, while avoiding the use of toxic organic solvents. Existing techniques that use this method are either prohibitively expensive, or are applicable to only a restricted set of taxa. Here we describe a cost-effective DNA extraction technique suitable for a wide range of plant and animal taxa that yields microgram quantities of high-molecular-weight genomic DNA at a throughput of 192 samples per day. Our technique is particularly robust for tissue samples that are insoluble or are rapidly discoloured or oxidized in standard DNA extraction buffers. We demonstrate the quality of DNA extracted using this method by applying the amplified fragment length polymorphism technique to plant species.
采用变性盐使 DNA 通过亲硫键可逆结合到硅基质上的 DNA 提取技术，可以从动植物组织中获得高质量的基因组 DNA，同时避免使用有毒的有机溶剂。现有的使用这种方法的技术要么非常昂贵，要么只适用于有限的分类群。在这里，我们描述了一种经济有效的 DNA 提取技术，适用于广泛的植物和动物分类群，每天可处理 192 个样本，产量为微克级的高分子量基因组 DNA。我们的技术对于在标准 DNA 提取缓冲液中不溶或迅速变色或氧化的组织样本特别稳健。我们通过将扩增片段长度多态性技术应用于植物物种来证明使用这种方法提取的 DNA 的质量。
