一种用于扩增片段长度多态性（AFLP）分析的大麻样本简单DNA提取方法。

A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis.

作者信息

Miller Coyle Heather, Shutler Gary, Abrams Sharon, Hanniman Janet, Neylon Suzanne, Ladd Carll, Palmbach Timothy, Lee Henry C

机构信息

Division of Scientific Services, Department of Public Safety, 278 Colony Street, Meriden, CT 06451, USA.

出版信息

J Forensic Sci. 2003 Mar;48(2):343-7.

PMID:12664992

Abstract

As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125-500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.

摘要

作为开发一种用于大麻样本个体化分子方法的第一步，我们评估了一种植物DNA提取试剂盒。QIAGEN植物DNeasy方法采用旋转柱形式回收DNA，对于从大麻的叶、花（芽）和种子样本中获得高分子量DNA是有效的。平均DNA产量为每100毫克新鲜植物组织125 - 500纳克。回收的DNA具有聚合酶链反应（PCR）质量，这是通过产生可重复的扩增片段长度多态性（AFLP）图谱的能力来衡量的。AFLP是一种用于创建植物品种DNA图谱的技术，作者正在将其应用于大麻样本，以关联克隆材料的种植者和经销商。QIAGEN植物DNeasy方法在将少量大麻处理成DNA方面简单、高效且可重复。