Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA, Department of Plant Pathology, North Dakota State University, Fargo, ND 55105, USA.
Mol Ecol Resour. 2008 Jul;8(4):910-2. doi: 10.1111/j.1755-0998.2008.02113.x.
Fourteen polymorphic microsatellite markers were developed for Mycosphaerella fijiensis, a fungus causing the black sigatoka disease in banana. The sequenced genome of M. fijiensis was screened for sequences with single sequence repeats (SSRs) using a Perl script. Fourteen SSR loci, evaluated on 48 M. fijiensis isolates from Hawaii, were identified to be highly polymorphic. These markers revealed two to 19 alleles, with an average of 6.43 alleles per locus. The estimated gene diversity ranged from 0.091 to 0.930 across the 14 microsatellite loci. The SSR markers developed would be useful for population genetics studies of M. fijiensis.
十四种多态性微卫星标记被开发用于研究引起香蕉黑条叶斑病的真菌——木贼镰刀菌。利用 Perl 脚本从木贼镰刀菌的测序基因组中筛选出单核苷酸重复序列（SSR）。在夏威夷的 48 个木贼镰刀菌分离株中评估了 14 个 SSR 位点，结果发现这些位点高度多态。这些标记显示出 2 到 19 个等位基因，每个位点的平均等位基因数为 6.43。在 14 个微卫星位点上，估计的基因多样性从 0.091 到 0.930 不等。开发的 SSR 标记将有助于木贼镰刀菌种群遗传学研究。
