Centre d'Ecologie Fonctionnelle et Evolutive, UMR 5175 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
Mol Ecol Resour. 2008 Sep;8(5):1062-4. doi: 10.1111/j.1755-0998.2008.02155.x. Epub 2008 Jul 8.
We describe 15 new variable microsatellites in the freshwater snail Aplexa (Physa) marmorata, as well as conditions for multiplexing and simultaneous genotyping sets of loci. Two to 11 alleles were detected per locus over the five populations studied, and up to eight alleles per locus and population. The observed heterozygosity was extremely low, most probably as the consequence of high selfing rates. The genetic differentiation among populations was large. These markers will constitute efficient tools for investigating the population structure in fragmented habitats. Cross-species amplification in three Physa species (P. acuta, P. fontinalis and P. gyrina) was rather unsuccessful.
我们描述了淡水蜗牛 Aplexa (Physa) marmorata 中的 15 个新的可变微卫星，以及用于多重和同时基因分型的条件。在所研究的五个种群中，每个位点检测到 2 到 11 个等位基因，每个位点和种群最多可检测到 8 个等位基因。观察到的杂合度极低，很可能是由于高自交率的结果。种群间的遗传分化很大。这些标记将成为研究破碎生境中种群结构的有效工具。在三个 Physa 物种（P. acuta、P. fontinalis 和 P. gyrina）中的跨物种扩增相当不成功。
