Gu Q H, Xiong B X, Zhu Y T, Wang Q, Shi P S
College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Genet Mol Res. 2012 May 18;11(2):1449-53. doi: 10.4238/2012.May.18.4.
Although it is a major freshwater gastropod species, genetic diversity of Bellamya aeruginosa was completely unknown. Eighteen microsatellite loci were isolated and characterized from (AC)(15)-enriched genomic libraries of the freshwater snail B. aeruginosa. Most of the 18 loci were successfully amplified and high polymorphic information content values were found, ranging from 0.244 to 0.792 (mean 0.541). The number of alleles per locus ranged from 5 to 13 (mean 8.8), the expected heterozygosity varied from 0.347 to 0.950 (mean 0.815) and the observed heterozygosity varied from 0.087 to 0.782 (mean 0.431). Eight loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni's correction and no significant genotypic linkage disequilibrium was detected between most locus pairs, except for TXH79-TXH97 and TXH113-TXH121. These 18 polymorphic microsatellite loci should be useful for population genetics analysis and species identification of Bellamya.
虽然铜锈环棱螺是一种主要的淡水腹足类物种，但其遗传多样性却完全未知。从铜锈环棱螺的（AC）(15)富集基因组文库中分离并鉴定了18个微卫星位点。这18个位点中的大多数都成功扩增，且发现具有较高的多态信息含量值，范围从0.244到0.792（平均0.541）。每个位点的等位基因数从5到13不等（平均8.8），期望杂合度从0.347到0.950不等（平均0.815），观察到的杂合度从0.087到0.782不等（平均0.431）。经Bonferroni校正后，有8个位点显著偏离哈迪-温伯格平衡，除TXH79 - TXH97和TXH113 - TXH121外，大多数位点对之间未检测到显著的基因型连锁不平衡。这18个多态微卫星位点应有助于铜锈环棱螺的群体遗传学分析和物种鉴定。
