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为热带木材树种轻木和破布木(Courbaril 和 stigonocarpa)分离和鉴定微卫星位点,并转移到这两个树种。

Isolation and characterization of microsatellite loci for Hymenaea courbaril and transferability to Hymenaea stigonocarpa, two tropical timber species.

机构信息

Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica (PqEB) Avenida W5 Norte (final), CP 02372, CEP 70770-900 Brasília, DF, Brazil, Universidade Estadual de Santa Cruz (UESC), CEP 45652-050 Ilhéus, BA, Brazil, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Caixa Postal 486, CEP 31270-901 Belo Horizonte, MG, Brazil.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1074-7. doi: 10.1111/j.1755-0998.2008.02159.x. Epub 2008 Jun 28.

DOI:10.1111/j.1755-0998.2008.02159.x
PMID:21585976
Abstract

Hymenaea courbaril is a tropical timber species, intensely exploited and found in the Amazon, Atlantic Forest and Brazilian Cerrado biome. Nine highly polymorphic microsatellite loci were developed from a genomic library enriched for AG/TC repeats. In a total of 41 individuals, from two natural populations, seven to 13 alleles per locus were detected and expected heterozygosity ranged from 0.75 to 0.90. Seven loci were effectively transferred to Hymenaea stigonocarpa. High levels of polymorphism make the present primers useful for population genetic studies and are a powerful tool to investigate mating system, gene flow and spatial genetic structure.

摘要

轻木是一种热带木材物种,主要分布于亚马逊、大西洋森林和巴西塞拉多生物群系,其资源正遭受过度开发。本研究从富含 AG/TC 重复序列的基因组文库中开发了 9 个高度多态性的微卫星位点。在来自两个自然种群的 41 个个体中,每个位点检测到 7 到 13 个等位基因,预期杂合度范围为 0.75 到 0.90。其中 7 个位点可有效转移到雨树属的 Hymenaea stigonocarpa 中。这些微卫星标记具有较高的多态性,可用于种群遗传研究,是研究交配系统、基因流和空间遗传结构的有力工具。

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