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从极度濒危的玳瑁(Eretmochelys imbricata)中分离和鉴定新型微卫星。

Isolation and characterization of novel microsatellites from the critically endangered hawksbill sea turtle (Eretmochelys imbricata).

机构信息

Department of Biology, University of Puerto Rico, PO Box 23360, San Juan, Puerto Rico 00931.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1098-101. doi: 10.1111/j.1755-0998.2008.02143.x. Epub 2008 Jul 18.

DOI:10.1111/j.1755-0998.2008.02143.x
PMID:21585983
Abstract

We isolated and characterized 12 microsatellite loci from the hawksbill sea turtle (Eretmochelys imbricata). The loci exhibited a variable number of alleles that ranged from three to 14 with an average observed heterozygosity of 0.70 (SD 0.18) across 40 hawksbill turtles from the Caribbean. The polymorphism exhibited individually and in combination makes them suitable for fine-scale genetic studies. In particular, the low probability of identity and high paternity exclusion of these markers makes them highly useful for parentage and relatedness studies. These new markers greatly increase the power of genetic studies directed towards the conservation of this endangered species.

摘要

我们从玳瑁(Eretmochelys imbricata)中分离并鉴定了 12 个微卫星位点。这些位点显示出可变数量的等位基因,范围从 3 到 14,在来自加勒比地区的 40 只玳瑁海龟中,平均观察到的杂合度为 0.70(标准差 0.18)。这些标记的个体和组合的多态性使其适合于精细遗传研究。特别是,这些标记的低同一性概率和高亲权排除率使得它们在亲子关系和亲缘关系研究中非常有用。这些新的标记大大提高了针对保护这一濒危物种的遗传研究的能力。

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