Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
State Key Laboratory of Marine Environmental Science and College of Ocean & Earth Sciences, Xiamen University, Xiamen, Fujian, China.
Sci Data. 2023 Sep 9;10(1):604. doi: 10.1038/s41597-023-02522-3.
Eretmochelys imbricata, a critically endangered sea turtle inhabiting tropical oceans and protected across the world, had an unknown genome sequence until now. In this study, we used HiFi reads and Hi-C technology to assemble a high-quality, chromosome-level genome of E. imbricata. The genome size was 2,138.26 Mb, with contig N50 length of 123.49 Mb and scaffold N50 of 137.21 Mb. Approximately 97.52% of the genome sequence was anchored to 28 chromosomes. A total of 20,206 protein-coding genes were predicted. We also analyzed the evolutionary relationships, gene family expansions, and positive selection of E. imbricata. Our results revealed that E. imbricata diverged from Chelonia mydas 38 million years ago and had enriched olfactory receptors and aging-related genes. Our genome will be useful for studying E. imbricata and its conservation.
玳瑁，一种生活在热带海洋的极危海龟，在全球范围内受到保护，其基因组序列直到现在仍是未知的。在这项研究中，我们使用 HiFi 读取和 Hi-C 技术组装了玳瑁的高质量、染色体水平基因组。基因组大小为 2138.26Mb，contig N50 长度为 123.49Mb，scaffold N50 为 137.21Mb。大约 97.52%的基因组序列被锚定在 28 条染色体上。共预测到 20206 个蛋白质编码基因。我们还分析了玳瑁的进化关系、基因家族扩张和正选择。我们的研究结果表明，玳瑁与蠵龟在 3800 万年前分化，并富集了嗅觉受体和与衰老相关的基因。我们的基因组将有助于研究玳瑁及其保护。
