Forest Research, Northern Research Station, Roslin EH25 9SY, UK.
Mol Ecol Resour. 2008 Nov;8(6):1340-3. doi: 10.1111/j.1755-0998.2008.02269.x.
Thirty-four novel microsatellite markers developed for wood cricket (Nemobius sylvestris) were tested and optimized. Twenty-five microsatellite loci were polymorphic, exhibiting between two and nine alleles. Observed heterozygosities ranged from 0.038 to 0.925. The microsatellites were also tested in a species belonging to another genus of the Gryllidae family (Gryllus bimaculatus). Two markers produced clear banding patterns with the expected product size. These markers will be used to study the effects of forest fragmentation on genetic connectivity using wood cricket as a model species.
为木蟋（Nemobius sylvestris）开发的 34 个新微卫星标记进行了测试和优化。25 个微卫星位点表现出多态性，具有 2 到 9 个等位基因。观察到的杂合度范围从 0.038 到 0.925。这些微卫星也在另一个蟋蟀科（Gryllidae）属（Gryllus bimaculatus）的物种中进行了测试。两个标记产生了与预期产物大小一致的清晰带型。这些标记将被用于研究森林破碎化对遗传连通性的影响，木蟋将作为模型物种。
