Centre for Ecology and Conservation, University of Exeter, Cornwall Campus, Penryn, Cornwall, TR10 9EZ, UK, NERC Molecular Genetics Facility (Sheffield), Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, South Yorkshire, S10 2TN, UK.
Mol Ecol Resour. 2008 Sep;8(5):1015-9. doi: 10.1111/j.1755-0998.2008.02139.x. Epub 2008 Jun 28.
We have developed a new set of 27 polymorphic markers for each of two cricket species, Gryllus bimaculatus and Gryllus campestris. Initially, 14 published G. bimaculatus loci were tested in G. campestris; however, only five loci were polymorphic. Therefore, we isolated an additional 50 new microsatellite loci from G. bimaculatus and tested these in both species. In a minimum of 20 individuals, 27 of the new loci were polymorphic in G. bimaculatus and 25 in G. campestris.
我们为两种蟋蟀物种，即双斑蟋蟀（Gryllus bimaculatus）和草地蟋蟀（Gryllus campestris），各开发了一整套 27 个多态性标记。最初，在草地蟋蟀中测试了 14 个已发表的双斑蟋蟀位点，但只有 5 个位点是多态性的。因此，我们从双斑蟋蟀中分离出另外 50 个新的微卫星位点，并在这两个物种中进行了测试。在至少 20 个个体中，27 个新位点在双斑蟋蟀中是多态性的，25 个在草地蟋蟀中是多态性的。
