开发圣卢西亚鬣蜥（Anolis luciae）的微卫星标记。

Development of microsatellite markers in the St Lucia anole, Anolis luciae.

机构信息

School of Biological Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd, LL57 2UW, UK.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1408-10. doi: 10.1111/j.1755-0998.2008.02206.x.

DOI:10.1111/j.1755-0998.2008.02206.x

Abstract

Anolis lizards are important models in studies of ecology and evolution. Here we describe 13 polymorphic microsatellites for use in population screening in the St Lucia anole, Anolis luciae, that can be used as a natural replicate to Anolis roquet on Martinique to study processes involved in population differentiation and speciation. Genotyping of 32 individuals using M13 tails and FAM-labelled universal M13 primers showed that all loci were polymorphic with high genetic diversity, averaging at 16.8 alleles per locus. Genotypic frequencies conformed to Hardy-Weinberg expectations, and there were no instances of linkage disequilibrium between loci.

摘要

安乐蜥是生态学和进化研究中的重要模型。在这里，我们描述了 13 个多态性微卫星，可用于圣卢西亚安乐蜥（Anolis luciae）的种群筛选，该物种可作为马提尼克岛罗奎安乐蜥（Anolis roquet）的自然复制品，用于研究种群分化和物种形成过程中涉及的问题。使用 M13 尾巴和 FAM 标记的通用 M13 引物对 32 个个体进行基因分型表明，所有位点均具有高度遗传多样性的多态性，平均每个位点 16.8 个等位基因。基因型频率符合哈迪-温伯格期望，并且没有发现位点之间的连锁不平衡。