用于研究美洲滨蟹的多态性核微卫星位点

Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

机构信息

School of Biological Sciences, Royal Holloway University of London, Egham TW20 0EX, UK.

出版信息

Mol Ecol Resour. 2008 May;8(3):653-5. doi: 10.1111/j.1471-8286.2007.02033.x.

DOI:10.1111/j.1471-8286.2007.02033.x

Abstract

Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.

摘要

从美洲滨蟹（Cancer pagurus L.）构建的微卫星富集基因组文库中分离出 12 个多态性微卫星 DNA 位点。对来自诺福克（英国）的 40 个个体进行基因分型，结果显示，每个位点的多态性水平不同，平均每个位点有 7.75 个等位基因（范围为 2-22）。每个位点的观察和预期杂合度分别在 0.025 到 0.868 和 0.025 到 0.947 之间。未检测到位点间连锁不平衡的证据，所有位点的基因型比例均符合哈迪-温伯格平衡预期。开发的微卫星位点构成了一套遗传标记，可应用于美洲滨蟹研究的众多领域。