Mokhtar M A A, Normah M N, Kumar S V, Baharum S N
Institute of Systems Biology, Universiti Kebangsaan Malaysia, Selangor, Malaysia.
Genet Mol Res. 2011 May 17;10(2):885-8. doi: 10.4238/vol10-2gmr1117.
Epinephelus fuscoguttatus is a commercially important marine fish species in southeast Asia. Due to overfishing and water pollution, this species has been declared as near-threatened. Thus, to provide information to help maintain and preserve the species, microsatellites were developed, using an enriched genomic library method. Thirty individuals were collected from the hatchery of the Fishery Research Institute, Terengganu, Malaysia. These individuals, from four to six years old, originated from Sabah and are maintained in captive culture as broodstock. Genomic DNA was extracted from the fins of selected individuals that weighed 3-8 kg. Ten microsatellite loci were found to be polymorphic in this population, with 5 to 21 alleles per locus. Observed and expected heterozygosities ranged from 0.53 to 0.97 and 0.59 to 0.95, respectively. Only one locus deviated significantly from Hardy-Weinberg equilibrium and no significant linkage disequilibrium was found among the pairs of loci. These polymorphic microsatellite loci will be used by the Malaysian Fishery Research Institute for investigating genetic diversity and for developing breeding strategies.
棕点石斑鱼是东南亚一种具有重要商业价值的海洋鱼类。由于过度捕捞和水污染，该物种已被宣布为近危物种。因此，为了提供有助于维持和保护该物种的信息，采用富集基因组文库法开发了微卫星。从马来西亚登嘉楼渔业研究所的孵化场收集了30个个体。这些个体年龄在4至6岁之间，原产于沙巴，作为亲鱼进行圈养。从体重3至8千克的选定个体的鳍中提取基因组DNA。在这个种群中发现10个微卫星位点具有多态性，每个位点有5至21个等位基因。观察到的杂合度和预期杂合度分别为0.53至0.97和0.59至0.95。只有一个位点显著偏离哈迪-温伯格平衡，并且在这些位点对之间未发现显著的连锁不平衡。马来西亚渔业研究所将使用这些多态性微卫星位点来研究遗传多样性并制定育种策略。
