Department of Marine Benthic Ecology and Evolution, Centre for Ecological and Evolutionary Studies, University of Groningen, Kerklaan 30, PO Box 14, 9750 AA Haren, the Netherlands Plant Evolutionary Ecology, Institute for Evolution and Biodiversity, University of Münster, D48149 Münster, Germany.
Mol Ecol Resour. 2008 Nov;8(6):1429-35. doi: 10.1111/j.1755-0998.2008.02251.x. Epub 2008 Aug 14.
We characterized 37 single nucleotide polymorphism (SNP) makers for eelgrass Zostera marina. SNP markers were developed using existing EST (expressed sequence tag)-libraries to locate polymorphic loci and develop primers from the functional expressed genes that are deposited in The ZOSTERA database (V1.2.1). SNP loci were genotyped using a single-base-extension approach which facilitated high-throughput genotyping with minimal optimization time. These markers show a wide range of variability among 25 eelgrass populations and will be useful for population genetic studies including evaluation of population structure, historical demography, and phylogeography. Potential applications include haplotype inference of physically linked SNPs and identification of genes under selection for temperature and desiccation stress.
我们对鳗草（Zostera marina）的 37 个单核苷酸多态性（SNP）标记进行了特征描述。使用现有的 EST（表达序列标签）文库，SNP 标记被开发出来，以定位多态性基因座，并从功能表达基因中开发引物，这些基因已被存入 ZOSTERA 数据库（V1.2.1）。SNP 基因座通过单碱基延伸方法进行基因分型，这种方法方便了高通量基因分型，且最小化了优化时间。这些标记在 25 个鳗草种群之间表现出广泛的变异性，将有助于进行种群遗传研究，包括评估种群结构、历史动态和系统地理学。潜在的应用包括物理连锁 SNP 的单倍型推断，以及对温度和干燥胁迫选择下的基因进行鉴定。
