Unit of Evolutionary Biology/Systematic Zoology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, Haus 26, D-14476 Potsdam, Germany, Wildlife Institute of India, Post Bag#18, Chandrabani, Dehradun, Uttaranchal 248001, India.
Mol Ecol Resour. 2008 Nov;8(6):1480-2. doi: 10.1111/j.1755-0998.2008.02292.x. Epub 2008 Aug 25.
We describe 11 dinucleotide and three tetranucleotide microsatellite loci for the critically endangered Indian tiger, Panthera tigris tigris. All of them were polymorphic with four to nine alleles per locus and an observed heterozygosity between 0.13 and 1.0. All primers also amplify microsatellite loci in leopard, Panthera pardus, and 12 primer pairs yielded reproducible results in domestic cat, Felis catus. These new microsatellites specifically developed for Indian tiger - in combination with those already available - comprise a reasonable number of loci to genetically analyse wild and captive populations of this illustrative species and might allow for recognition of individual tigers.
我们描述了 11 个二核苷酸和 3 个四核苷酸微卫星位点,用于濒危的印度虎, Panthera tigris tigris。所有这些位点都是多态的,每个位点有 4 到 9 个等位基因,观测杂合度在 0.13 到 1.0 之间。所有引物也能扩增豹, Panthera pardus 的微卫星位点,并且 12 对引物在家猫, Felis catus 中产生可重复的结果。这些专门为印度虎开发的新微卫星与现有的微卫星相结合,构成了对该典型物种的野生和圈养种群进行遗传分析的合理数量的位点,并且可能允许识别个体老虎。