大西洋鳕鱼（Gadus morhua L.）18 个新微卫星位点的特征分析。
Characterization of 18 new microsatellite loci in Atlantic cod (Gadus morhua L.).
机构信息
Matis-Prokaria, Gylfaflöt 5, 112 Reykjavik, Iceland, Marine Research Institute, Skulagata 4, 101 Reykjavik, Iceland, Department of Biology, University of Iceland, Sturlugata 7, 101 Reykjavik, Iceland.
出版信息
Mol Ecol Resour. 2008 Nov;8(6):1503-5. doi: 10.1111/j.1755-0998.2008.02327.x.
Eighteen new microsatellite loci consisting of 10 di-, 5 tri-, 2 tetra- and 1 heptanucleotide repeats are introduced for the Atlantic cod (Gadus morhua L.). All loci were co-amplified in two polymerase chain reactions (plus two previously published microsatellites) and all products were typed clearly. The number of alleles per locus ranged from six (PGmo130) to 45 (PGmo76) and the observed heterozygosity ranged from 0.356 (PGmo130) to 0.957 (PGmo95). All loci except one followed Hardy-Weinberg expectations. Genetic linkage disequilibrium analysis between all pairs of loci did not yield any significant values.
介绍了 18 个新的微卫星位点，由 10 个二核苷酸、5 个三核苷酸、2 个四核苷酸和 1 个七核苷酸重复组成，用于大西洋鳕鱼（Gadus morhua L.）。所有位点都在两个聚合酶链反应（加上两个以前发表的微卫星）中共同扩增，所有产物都能清晰地进行分型。每个位点的等位基因数从 6 个（PGmo130）到 45 个（PGmo76）不等，观察到的杂合度从 0.356（PGmo130）到 0.957（PGmo95）不等。除了一个位点外，所有位点都符合哈迪-温伯格期望。对所有位点之间的所有对进行遗传连锁不平衡分析，没有得到任何显著值。
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