Department of Biobehavioral Nursing and Health Systems, University of Washington, Seattle, WA, USA.
Biol Res Nurs. 2011 Jul;13(3):235-42. doi: 10.1177/1099800411402056. Epub 2011 May 17.
Recently, biobehavioral nursing scientists have focused their attention on the search for biomarkers or biological signatures to identify patients at risk for various health problems and poor disease outcomes. In response to the national impetus for biomarker discovery, the measurement of biological fluids and tissues has become increasingly sophisticated. Urine proteomics, in particular, may hold great promise for biobehavioral focused nursing scientists for examination of symptom-and syndrome-related research questions. Urine proteins are easily accessible secreted proteins that provide direct and indirect windows into bodily functions. Advances in proteomics and biomarker discovery provide new opportunities to conduct research studies with banked and fresh urine to benefit diagnosis, prognosis, and evaluation of outcomes in various disease populations. This article provides a review of proteomics and a rationale for utilizing urine proteomics in biobehavioral research. It addresses as well some of the challenges involved in data collection and sample preparation.
最近，生物行为护理科学家将注意力集中在寻找生物标志物或生物学特征上，以识别有各种健康问题和不良疾病结局风险的患者。为了响应国家对生物标志物发现的推动，生物体液和组织的测量变得越来越复杂。尿液蛋白质组学，特别是对于生物行为护理科学家来说，可能为检查症状和综合征相关的研究问题提供了巨大的希望。尿液蛋白是容易获得的分泌蛋白，为身体功能提供了直接和间接的窗口。蛋白质组学和生物标志物发现的进步为使用储存和新鲜尿液进行研究提供了新的机会，以有益于各种疾病人群的诊断、预后和结果评估。本文综述了蛋白质组学，并提出了在生物行为研究中利用尿液蛋白质组学的基本原理。它还涉及到数据收集和样品制备中涉及的一些挑战。
