Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur, India.
Nanotechnology. 2011 Jul 1;22(26):265505. doi: 10.1088/0957-4484/22/26/265505. Epub 2011 May 18.
Fe(3)O(4) nanoparticles covalently linked to a gold electrode have been used for immobilizing catalase (CAT) enzyme to sense the presence of various concentrations of H(2)O(2). These nanoparticles ranging from 20 to 30 nm were synthesized by thermal co-precipitation of ferric and ferrous chlorides. SEM and XRD have been used for morphological and structural characterization of Fe(3)O(4) nanoparticles. CAT enzyme was linked covalently to the surface of iron oxide using carbodiimide in phosphate buffer (pH 7.4) at 4 °C. The enzyme-iron oxide link was confirmed by FT-IR spectroscopy. Sensing studies carried out using cyclic voltammetry showed a linear response of the CAT/nano Fe(3)O(4)/Au bioelectrode towards H(2)O(2) between 1.5 and 13.5 µM with a very sharp response time of 2 s.
通过热共沉淀法合成了粒径为 20 至 30nm 的四氧化三铁纳米粒子，并将其共价连接到金电极上，用于固定过氧化氢酶（CAT）酶以检测不同浓度的 H2O2。使用扫描电子显微镜（SEM）和 X 射线衍射（XRD）对 Fe3O4 纳米粒子进行了形态和结构表征。CAT 酶在磷酸盐缓冲液（pH 7.4）中于 4°C 下使用碳二亚胺共价连接到氧化铁表面。通过傅里叶变换红外光谱（FT-IR）对酶-氧化铁键进行了确认。使用循环伏安法进行的传感研究表明，CAT/纳米 Fe3O4/Au 生物电极对 1.5 至 13.5µM 之间的 H2O2 具有线性响应，响应时间非常快，仅为 2 秒。
