Martel P, Houdebine L M
INRA, Laboratoire des Sciences de la Consommation, Jouy-en-Josas, France.
Reprod Nutr Dev. 1990;30(1):85-90. doi: 10.1051/rnd:19900108.
Amiloride, an inhibitor of Na+/H+ exchange, was added at various concentrations to the culture medium of rabbit mammary explants. In the concentration range 100-250 microM, amiloride progessively inhibited 14C-thymidine incorporation induced by insulin, EGF or prolactin. Up to 250 microM, amiloride, which did not inhibit basal protein synthesis, was not cytotoxic, but it reduced basal DNA synthesis at the highest concentration. Addition of amiloride to the culture medium of mammary explants also strongly inhibited the induction of casein synthesis and casein mRNA accumulation by prolactin. The inhibition by amiloride is therefore not specific of the mitogenic action of prolactin since this drug also prevented its lactogenic action. The data reported here describe a new inhibitory action of amiloride on the transmission of the lactogenic signals.
氨氯吡咪是一种Na+/H+交换抑制剂，将其以不同浓度添加到兔乳腺外植体的培养基中。在100 - 250微摩尔的浓度范围内，氨氯吡咪逐渐抑制胰岛素、表皮生长因子（EGF）或催乳素诱导的14C - 胸腺嘧啶核苷掺入。在高达250微摩尔时，氨氯吡咪不抑制基础蛋白合成，也无细胞毒性，但在最高浓度时会降低基础DNA合成。将氨氯吡咪添加到乳腺外植体培养基中还强烈抑制催乳素诱导的酪蛋白合成和酪蛋白mRNA积累。因此，氨氯吡咪的抑制作用并非催乳素促有丝分裂作用所特有的，因为这种药物也阻止了其催乳作用。此处报道的数据描述了氨氯吡咪对催乳信号传递的一种新的抑制作用。
