大肠杆菌中smp'-lacZ基因融合体的IS30激活
IS30 activation of an smp'-lacZ gene fusion in Escherichia coli.
作者信息
Neuwald A F, Stauffer G V
机构信息
Department of Microbiology, University of Iowa, Iowa City 52242.
出版信息
FEMS Microbiol Lett. 1990 Mar 1;56(1-2):13-7. doi: 10.1016/0378-1097(90)90116-8.
PMID:2158922
Abstract
The Escherichia coli serB gene is divergently transcribed from the gene. Six independently isolated IS30 insertions within the 5' end of the serB structural gene resulted in increased expression of an smp'-lacZ gene fusion. DNA sequence analysis of two of the insertions suggests that a promoter was created upon IS30 insertion.
摘要
大肠杆菌serB基因与该基因呈反向转录。在serB结构基因5'端内六个独立分离的IS30插入导致smp'-lacZ基因融合体的表达增加。对其中两个插入进行的DNA序列分析表明，IS30插入后产生了一个启动子。
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