Coyne M S, Arunakumari A, Pankratz H S, Tiedje J M
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
J Bacteriol. 1990 May;172(5):2558-62. doi: 10.1128/jb.172.5.2558-2562.1990.
The locations of cytochrome cd1 nitrite reductases in Pseudomonas aeruginosa and Pseudomonas fluorescens and copper nitrite reductases in Achromobacter cycloclastes and Achromobacter xylosoxidans were identified. Immunogold labeling with colloidal-gold probes showed that the nitrite reductases were synthesized exclusively in anaerobically grown (denitrifying) cells. Little immunogold label occurred in the cytoplasm of these four strains; most was found in the periplasmic space or was associated with cell membranes. Immunogold labeling of thin sections was superior to fractionation by osmotic shock for locating nitrite reductases. The results support models of dentrification energetics that require a periplasmic, not a cytoplasmic, location for nitrite reductases.
确定了铜绿假单胞菌和荧光假单胞菌中细胞色素cd1亚硝酸还原酶的位置，以及环裂无色杆菌和木糖氧化无色杆菌中铜亚硝酸还原酶的位置。用胶体金探针进行免疫金标记显示，亚硝酸还原酶仅在厌氧生长（反硝化）的细胞中合成。这四种菌株的细胞质中几乎没有免疫金标记；大部分位于周质空间或与细胞膜相关。对于亚硝酸还原酶的定位，薄切片的免疫金标记优于渗透压休克分级分离法。这些结果支持反硝化能量学模型，该模型要求亚硝酸还原酶位于周质而非细胞质中。
