Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.
Chem Soc Rev. 2011 Dec;40(12):5789-801. doi: 10.1039/c1cs15054e. Epub 2011 May 17.
The self-assembly of nanosized DNA templates--based on formation of duplex, triplex, quadruplex or even pentaplex structures--provides unique opportunities for the controlled presentation of appended functional units. Recently, researchers have recognized the potential of such DNA scaffolds to address questions in the life sciences. In this critical review the focus is on the exploration of proteins. It is shown how different scaffolds can be used to control localization, structure and bioactivity of proteins and protein ligands. Further examples demonstrate that DNA-based recognition can even be used to trigger the formation of protein targeted molecules. Potential and existing applications in protein detection, drug discovery, structural characterization of protein targets as well as in the design of nucleic acid responsive pharmacophores are discussed (107 references).
纳米级 DNA 模板的自组装——基于双链体、三链体、四链体甚至五链体结构的形成——为附加功能单元的可控呈现提供了独特的机会。最近，研究人员已经认识到这种 DNA 支架在解决生命科学问题方面的潜力。在这篇评论中，重点是对蛋白质的探索。展示了不同的支架如何用于控制蛋白质和蛋白质配体的定位、结构和生物活性。进一步的例子表明，基于 DNA 的识别甚至可以用于触发靶向蛋白质分子的形成。讨论了其在蛋白质检测、药物发现、蛋白质靶标的结构特征以及核酸响应药效团设计中的潜在和现有应用（107 篇参考文献）。
