Investigation of transport and unpacking mechanisms of polyplexes for transfection efficacy on different cell lines.
作者信息
Trusov G A, Ulasov A V, Beletkaia E A, Khramtsov Yu V, Durymanov M O, Rosenkranz A A, Sverdlov E D, Sobolev A S
机构信息
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia.
出版信息
Dokl Biochem Biophys. 2011 Mar-Apr;437(1):77-9. doi: 10.1134/S1607672911020062. Epub 2011 May 18.
Abstract
摘要
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2
Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET.通过基于量子点-荧光共振能量转移构建的模型对多聚体的细胞内解包动力学进行定量比较。
Mol Ther. 2008 Feb;16(2):324-32. doi: 10.1038/sj.mt.6300392. Epub 2008 Jan 8.
3
Nano-carriers for DNA delivery to the lung based upon a TAT-derived peptide covalently coupled to PEG-PEI.基于与聚乙二醇-聚乙烯亚胺共价偶联的源自TAT的肽的用于将DNA递送至肺部的纳米载体。
J Control Release. 2005 Dec 5;109(1-3):299-316. doi: 10.1016/j.jconrel.2005.09.036. Epub 2005 Nov 17.
4
Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer.质粒DNA在细胞质中的代谢不稳定性：基因转移的潜在障碍。
Gene Ther. 1999 Apr;6(4):482-97. doi: 10.1038/sj.gt.3300867.
5
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