Grabherr Manfred G, Mauceli Evan, Ma Li-Jun
University of Massachusetts, Amkebt, MA, USA.
Methods Mol Biol. 2011;722:1-9. doi: 10.1007/978-1-61779-040-9_1.
Decoding the genome sequence is becoming a fundamental tool for molecular, genetic, and genomic studies. This chapter reviews the history of DNA sequencing and technical principles of different sequencing platforms, and compares the strengths and weaknesses of different techniques for high-throughput genome sequencing applications are compared. It also covers brief descriptions on genome assembly and its validation.
解码基因组序列正成为分子、遗传和基因组研究的一项基础工具。本章回顾了DNA测序的历史以及不同测序平台的技术原理，并比较了不同高通量基因组测序技术的优缺点。本章还简要介绍了基因组组装及其验证。
