Peng Jianglong, Zhou Ying, Jia Haoyuan, Li Ling, Qian Jun, Han Feifei, Yin Hao, Cui Yubao
School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, China.
Department of Pediatrics Laboratory, Wuxi Children's Hospital, Wuxi, China.
J Insect Sci. 2018 Sep 1;18(5):11. doi: 10.1093/jisesa/iey099.
Aquaporin water channel proteins are highly conserved across many diverse species. Some evidence indicates that aquaporins in insects may contribute to insect-related mammalian diseases and inflammation, and thus these proteins may represent viable therapeutic targets. Here, we used RNA sequencing and bioinformatics to identify putative aquaporins from the house dust mite, Dermatophagoides farinae. Six putative aquaporins were identified based on sequence similarity with aquaporins from other species. These putative aquaporins, deposited in GenBank and named DerfAQP1-4 (KY231248, KY231249, KY231250, and KY231251, respectively), DerfAQP5.01, and DerfAQP5.02 (KY231252 and KY231253), were successfully cloned into a bacterial plasmid. The identification of full-length aquaporin sequences from D. farinae provides a foundation for future molecular and biochemical studies of these proteins in D. farinae and related species.
水通道蛋白在许多不同物种中高度保守。一些证据表明，昆虫体内的水通道蛋白可能与昆虫相关的哺乳动物疾病和炎症有关，因此这些蛋白质可能是可行的治疗靶点。在这里，我们使用RNA测序和生物信息学从屋尘螨（粉尘螨）中鉴定推定的水通道蛋白。基于与其他物种水通道蛋白的序列相似性，鉴定出了六种推定的水通道蛋白。这些推定的水通道蛋白已存入GenBank，并分别命名为DerfAQP1 - 4（分别为KY231248、KY231249、KY231250和KY231251）、DerfAQP5.01和DerfAQP5.02（KY231252和KY231253），它们已成功克隆到细菌质粒中。从粉尘螨中鉴定全长水通道蛋白序列为今后对这些蛋白在粉尘螨及相关物种中的分子和生化研究奠定了基础。
