Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Mar Biotechnol (NY). 2012 Feb;14(1):1-9. doi: 10.1007/s10126-011-9383-6. Epub 2011 May 19.
For genetic analysis and linkage mapping of bay scallop (Argopecten irradians), a set of 120 novel simple sequence repeat markers were developed from microsatellite-enriched libraries and expressed sequence tags. An inter-subspecies hybrid bay scallop family (CC5) of 46 progeny was analyzed as the reference population to confirm polymorphism and test the segregation patterns of these loci. A total of 104 microsatellite markers were polymorphic in the reference family, among which 36 in female, 28 in male, and 40 in both parents, respectively. Linkage analysis allowed mapping these markers to 15 linkage groups, which is close to the haploid chromosome number of bay scallop (n = 16). Analysis of the 40 markers segregating in both parents showed a higher recombination rate in the female parent, with the average of female-to-male recombination ratio of 1.09:1 between linked pairs of markers. When null alleles were considered, there were 17 loci showing segregation distortion at the 5% significance level using the chi-square test. The microsatellite markers developed in this study provide a useful resource for future linkage mapping and quantitative loci analysis in A. irradians.
为了对海湾扇贝（Argopecten irradians）进行遗传分析和连锁图谱绘制，本研究从微卫星富集文库和表达序列标签中开发了一套 120 个新的简单重复序列标记。利用一个由 46 个子代组成的种间杂交海湾扇贝（CC5）家系作为参考群体，对这些标记进行多态性检测和分离模式分析。在参考家系中，104 个微卫星标记具有多态性，其中母本中有 36 个，父本中有 28 个，双亲中各有 40 个。连锁分析将这些标记定位到 15 个连锁群，这与海湾扇贝的单倍体染色体数（n=16）相近。对 40 个在双亲中分离的标记进行分析表明，母本的重组率更高，连锁标记之间的雌性到雄性重组比平均为 1.09:1。当考虑无效等位基因时，17 个标记在卡方检验中达到 5%的显著水平，表现出分离扭曲。本研究开发的微卫星标记为未来海湾扇贝的连锁图谱绘制和数量性状位点分析提供了有用的资源。
