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两个大西洋海湾扇贝亚种的基因组草案:海湾扇贝和同心海湾扇贝。

Draft genomes of two Atlantic bay scallop subspecies Argopecten irradians irradians and A. i. concentricus.

机构信息

School of Fishery, Zhejiang Ocean University, Zhoushan, 316022, China.

Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.

出版信息

Sci Data. 2020 Mar 23;7(1):99. doi: 10.1038/s41597-020-0441-7.

Abstract

The two subspecies of Atlantic bay scallop (Argopecten irradians), A. i. irradians and A. i. concentricus, are economically important aquacultural species in northern and southern China. Here, we performed the whole-genome sequencing, assembly, and gene annotation and produced draft genomes for both subspecies. In total, 253.17 and 272.97 gigabases (Gb) of raw reads were generated from Illumina Hiseq and PacBio platforms for A. i. irradians and A. i. concentricus, respectively. Draft genomes of 835.7 Mb and 874.82 Mb were assembled for the two subspecies, accounting for 83.9% and 89.79% of the estimated sizes of their corresponding genomes, respectively. The contig N50 and scaffold N50 were 78.54 kb and 1.53 Mb for the A. i. irradians genome, and those for the A. i. concentricus genome were 63.73 kb and 1.25 Mb. Moreover, 26,777 and 25,979 protein-coding genes were predicted for A. i. irradians and A. i. concentricus, respectively. These valuable genome assemblies lay a solid foundation for future theoretical studies and provide guidance for practical scallop breeding.

摘要

大西洋海湾扇贝(Argopecten irradians)的两个亚种,A. i. irradians 和 A. i. concentricus,是中国北方和南方具有重要经济价值的水产养殖品种。在这里,我们进行了全基因组测序、组装和基因注释,并为这两个亚种生成了基因组草图。分别从 Illumina Hiseq 和 PacBio 平台为 A. i. irradians 和 A. i. concentricus 生成了 253.17 和 272.97 千兆碱基(Gb)的原始读数。组装了 835.7 Mb 和 874.82 Mb 的基因组草图,分别占其相应基因组估计大小的 83.9%和 89.79%。A. i. irradians 基因组的 contig N50 和 scaffold N50 分别为 78.54 kb 和 1.53 Mb,而 A. i. concentricus 基因组的分别为 63.73 kb 和 1.25 Mb。此外,分别预测了 A. i. irradians 和 A. i. concentricus 的 26777 和 25979 个蛋白质编码基因。这些有价值的基因组组装为未来的理论研究奠定了坚实的基础,并为实际的扇贝养殖提供了指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e7d/7090048/adc353dd512d/41597_2020_441_Fig1_HTML.jpg

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