Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China.
Mar Biotechnol (NY). 2013 Jun;15(3):275-312. doi: 10.1007/s10126-012-9485-9. Epub 2012 Oct 17.
Common carp (Cyprinus carpio L.) is cultured worldwide and is a major contributor to the world's aquaculture production. The common carp has a complex tetraploidized genome, which may historically experience additional whole genome duplication than most other Cyprinids. Fine maps for female and male carp were constructed using a mapping panel containing one F1 family with 190 progeny. A total of 1,025 polymorphic markers were used to construct genetic maps. For the female map, 559 microsatellite markers in 50 linkage groups cover 3,468 cM of the genome. For the male map, 383 markers in 49 linkage groups cover 1,811 cM of the genome. The consensus map was constructed by integrating the new map with two published linkage maps, containing 732 markers and spanning 3,278 cM in 50 linkage groups. The number of consensus linkage groups corresponds to the number of common carp chromosomes. A significant difference on sex recombinant rate was observed that the ratio of female and male recombination rates was 4.2:1. Comparative analysis was performed between linkage map of common carp and genome of zebrafish (Danio rerio), which revealed clear 2:1 relationship of common carp linkage groups and zebrafish chromosomes. The results provided evidence that common carp did experienced a specific whole genome duplication event comparing with most other Cyprinids. The consensus linkage map provides an important tool for genetic and genome study of common carp and facilitates genetic selection and breeding for common carp industry.
鲤鱼（Cyprinus carpio L.）在全球范围内养殖，是世界水产养殖产量的主要贡献者。鲤鱼具有复杂的四倍体基因组，其历史上可能经历了比大多数其他鲤科鱼类更多的全基因组复制。使用包含 190 个后代的 F1 家族的图谱构建面板，为雌性和雄性鲤鱼构建了精细图谱。总共使用了 1025 个多态性标记来构建遗传图谱。对于雌性图谱，50 个连锁群中的 559 个微卫星标记覆盖基因组的 3468 厘摩。对于雄性图谱，49 个连锁群中的 383 个标记覆盖基因组的 1811 厘摩。通过将新图谱与包含 732 个标记且跨越 50 个连锁群的 3278 厘摩的两个已发表的图谱整合，构建了共识图谱。共识连锁群的数量与鲤鱼染色体的数量相对应。观察到性别重组率存在显著差异，雌性和雄性重组率的比值为 4.2:1。对鲤鱼的连锁图谱和斑马鱼（Danio rerio）的基因组进行了比较分析，结果显示鲤鱼的连锁群与斑马鱼的染色体之间存在明显的 2:1 关系。这些结果为鲤鱼与大多数其他鲤科鱼类相比经历了特定的全基因组复制事件提供了证据。共识连锁图谱为鲤鱼的遗传和基因组研究提供了重要工具，并为鲤鱼产业的遗传选择和育种提供了便利。
